Primer

Part:BBa_K923002:Design

Designed by: Achmad Farajallah   Group: iGEM12_BAU-Indonesia   (2012-09-26)


Reverse Primer for Thermobifida fusca cutinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reverse primer Annealing temperature: 65 C Temperature melting (TM) : 70 C


Source

This primer designed from Thermobifida fusca cutinase, the sequence was taken from gene bank

References